epc-2 cells  (Corning Life Sciences)

Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 1. EPC morphology and gene expression. (A) Appearance of a freshly isolated EPC colony at passage 0 on day 9 of culture. (B) Appearance of EPCs in culture at passage 2, (C–F) EPCs express the endothelial cell specific markers CD31 (C, red, DAPI blue) and CD144 (D, green, DAPI blue) and pluripotency associated markers NANOG (E, red) and REX-1 (F, red, DAPI blue). Images A-B at 50X magnification, images C, D, E, F at 100X magnification.

Article Snippet: Transition from EPC culture medium (Cat. no. C-22111, Promocell, via VWR, Leicester, UK) to pluripotency medium (KSR, previously described in [17,18]) was attempted at days 3, 5, 7, 14 and 21.

Techniques: Gene Expression, Isolation

Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 2. Generation and characterization of EPC-srRNA-iPSCs. (A) Timeline for the reprogramming of human EPCs using srRNA. (B) EPC-srRNA-iPSCs express pluripotency markers. (C) In vivo teratoma analysis of EPC-srRNA- iPSCs demonstrate trilineage germ layer differentiation potential. (40x magnification for all micrographs).

Article Snippet: Transition from EPC culture medium (Cat. no. C-22111, Promocell, via VWR, Leicester, UK) to pluripotency medium (KSR, previously described in [17,18]) was attempted at days 3, 5, 7, 14 and 21.

Techniques: In Vivo

Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 3. Reprogramming of human AB serum-derived EPC-iPSC using non- modified RNA. (A) Timeline for the reprogramming of human EPCs using the non-modified RNA. 1 × 105 EPCs were seeded onto iMatrix-511 in EPC-Reprogramming Medium containing 10 % human serum. On day 10, culture medium was transitioned to NutriStem hPSC XF Medium. (B) Primary reprogramming cul ture morphology progression, resulting from the reprogramming of EPCs with non-modified RNA on iMatrix-511 and EPC-Reprogramming medium contain ing human serum. Day 6, 8, 13 primary EPC-RNA-iPS cell colonies were iden tified using StainAlive TRA-1-60 antibody and are able to be isolated from the primary culture by Day 12−14. (C) EPC-RNA-iPSCs were expanded on iMatrix- 511 and stained for pluripotency associated genes at P6 by immunostaining. (D) In vitro differentiation of P8 EPC-RNA-iPSCs expanded on iMatrix-511 were differentiated into early endoderm (AFP in red), neuronal cells (nestin in red; b- tubulin in green) and cardiomyocytes (Troponin T in red), DAPI (blue). (E) Histological analysis of teratoma resulting from the injection of EPC-iPS cells (p13) into the kidney capsule of NOD-SCID mice. Images in (B) 100X magnification, images in (C-D) at 50X magnification and e 40X magnification.

Article Snippet: Transition from EPC culture medium (Cat. no. C-22111, Promocell, via VWR, Leicester, UK) to pluripotency medium (KSR, previously described in [17,18]) was attempted at days 3, 5, 7, 14 and 21.

Techniques: Derivative Assay, Modification, Isolation, Staining, Immunostaining, In Vitro, Injection

Journal: New biotechnology

Article Title: Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications.

doi: 10.1016/j.nbt.2021.02.001

Figure Lengend Snippet: Fig. 4. Characterization and differentiation of autologous serum-derived srRNA-EPC-iPSCs. (A) Autologous serum-derived srRNA-EPC-iPSCs express pluripotency markers. (B) Autologous serum derived srRNA-EPC-iPSCs can be differentiated into all three germ layers in vitro. All images at 100X magnification.

Article Snippet: Transition from EPC culture medium (Cat. no. C-22111, Promocell, via VWR, Leicester, UK) to pluripotency medium (KSR, previously described in [17,18]) was attempted at days 3, 5, 7, 14 and 21.

Techniques: Derivative Assay, In Vitro